strogen is a pleiotropic hormone that actsbeyond the scope of its reproductive functions andexerts protective actions on multiple tissues includingthe brain. The protective actions of estrogen carrytremendous implications for the promotion of healthand the prevention of disease in postmenopausalwomen. Since the life span of women has increased fromapproximately 50 to 80 years, but the age of themenopause remains at about 51 years, women may nowlive over three decades of their lives in a hypoestrogenic,postmenopausal state. The impact of prolonged hypo-estrogenicity is now a critical health concern, since werealize that these women may suffer from an increasedvulnerability to a variety of diseases. Conversely, replace-ment with estrogen appears to act in the primary pre-vention of many disease processes, including neuro-degeneration. Estrogen, however, is not always beneficial,as high and unopposed levels may increase the risk forcertain cancers in some women. Our challenge, therefore,is to design hormone replacement therapies that exertonly beneficial effects in the body. To this end, we mustgain a more complete understanding of the spectrum ofestrogens actions and, more specifically, we must dissectthe mechanisms that underlie its actions.The broad spectrum of estrogens actions includes sig-nificant protection of the brain and primary preventionagainst neurodegeneration. Clinical observations indi-cate that estrogen replacement in postmenopausalwomen can (i) ameliorate cognitive dysfunction, and (ii)decrease the risk and delay the onset of degenerativeconditions such as Alzheimers disease (AD) and stroke.1 4 9B a s i c r e s e a r c hWe now appreciate that estrogen is a pleiotropic gonadalsteroid that exerts profound effects on the plasticity andcell survival of the adult brain. Over the past century, thelife span of women has increased, but the age of themenopause remains constant. This means that womenmay now live over one third of their lives in a hypoestro-genic, postmenopausal state. The impact of prolongedhypoestrogenicity on the brain is now a critical healthconcern as we realize that these women may suffer anincreased risk of cognitive dysfunction and neurodegen-eration due to a variety of diseases. Accumulating evi-dence from both clinical and basic science studies indicatesthat estrogen exerts critical protective actions against neuro-degenerative conditions such as Alzheimers disease andstroke. Here, we review the discoveries that comprise ourcurrent understanding of estrogen action against neuro-degeneration. These findings carry far-reaching possibili-ties for improving the quality of life in our aging popula-tion.Dialogues Clin Neurosci. 2002;4:149-162.Estrogen and neuroprotection: from clinicalobservations to molecular mechanismsDena B. Dubal, PhD; Phyllis M. Wise, PhDKeywords: estrogen; estradiol; estrogen replacement therapy; menopause; stroke;cerebral ischemia; Alzheimers disease; cognition, brain injury; neuroprotectionAuthor affiliations: Department of Physiology, University of Kentucky Collegeof Medicine, Lexington, KY, USA (Dena B. Dubal, PhD); Division of BiologicalSciences, University of California Davis, Davis, Calif, USA (Phyllis M. Wise, PhD)Address for correspondence: Dr Phyllis M. Wise, Dean, Division ofBiological Sciences University of California Davis, One Shields Avenue,Davis, CA 95616-8536, USA(e-mail: pmwise@ucdavis.edu)E